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Antiviral Activity of Fritillaria thunbergii Extract against Human Influenza Virus H1N1 (PR8) In Vitro, In Ovo and In Vivo

Journal of Microbiology and Biotechnology 2020년 30권 2호 p.172 ~ 177
 ( Kim Min-Jee ) - Konkuk University Department of Biomedical Science and Engineering

 ( Nguyen Dinh-Van ) - Konkuk University Department of Biomedical Science and Engineering
 ( Heo Yoon-Ki ) - Konkuk University Department of Biomedical Science and Engineering
박기훈 ( Park Ki-Hoon ) - Konkuk University Department of Biomedical Science and Engineering
백현동 ( Paik Hyun-Dong ) - Konkuk University Department of Food Science and Biotechnology of Animal Resources
김영봉 ( Kim Young-Bong ) - Konkuk University Department of Biomedical Science and Engineering

Abstract


Influenza viruses cause respiratory diseases in humans and animals with high morbidity and mortality rates. Conventional anti-influenza drugs are reported to exert side effects and newly emerging viral strains tend to develop resistance to these commonly used agents. Fritillaria thunbergii (FT) is traditionally used as an expectorant for controlling airway inflammatory disorders. Here, we evaluated the therapeutic effects of FT extracts against influenza virus type A (H1N1) infection in vitro, in ovo, and in vivo. In the post-treatment assay, FT extracts showed high CC50 (7,500 μg/ml), indicating low toxicity, and exerted moderate antiviral effects compared to oseltamivir (SI 50.6 vs. 222) in vitro. Antiviral activity tests in ovo revealed strong inhibitory effects of both FT extract and oseltamivir against H1N1 replication in embryonated eggs. Notably, at a treatment concentration of 150 mg/kg, only half the group administered oseltamivir survived whereas the FT group showed 100% survival, clearly demonstrating the low toxicity of FT extracts. Consistent with these findings, FT-administered mice showed a higher survival rate with lower body weight reduction relative to the oseltamivir group upon treatment 24 h after viral infection. Our collective results suggest that FT extracts exert antiviral effects against influenza H1N1 virus without inducing toxicity in vitro, in ovo or in vivo, thereby supporting the potential utility of FT extract as a novel candidate therapeutic drug or supplement against influenza.

키워드

Antiviral activity; Influenza A (H1N1); Fritillaria thunbergii; oseltamivir
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