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Cold-Stress Response of Probiotic Lactobacillus plantarum K25 by iTRAQ Proteomic Analysis

Journal of Microbiology and Biotechnology 2020년 30권 2호 p.187 ~ 195
 ( Liu Shaoli ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health

 ( Ma Yimiao ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health
 ( Zheng Yi ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health
 ( Zhao Wen ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health
 ( Zhao Xiao ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health
 ( Luo Tianqi ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health
 ( Zhang Jian ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health
 ( Yang Zhennai ) - Beijing Technology and Business University Beijing Advanced Innovation Center for Food Nutrition and Human Health

Abstract


To understand the molecular mechanism involved in the survivability of cold-tolerant lactic acid bacteria was of great significance in food processing, since these bacteria play a key role in a variety of low-temperature fermented foods. In this study, the cold-stress response of probiotic Lactobacillus plantarum K25 isolated from Tibetan kefir grains was analyzed by iTRAQ proteomic method. By comparing differentially expressed (DE) protein profiles of the strain incubated at 10oC and 37oC, 506 DE proteins were identified. The DE proteins involved in carbohydrate, amino acid and fatty acid biosynthesis and metabolism were significantly down-regulated, leading to a specific energy conservation survival mode. The DE proteins related to DNA repair, transcription and translation were up-regulated, implicating change of gene expression and more protein biosynthesis needed in response to cold stress. In addition, two-component system, quorum sensing and ABC (ATP-binding cassette) transporters also participated in cell cold-adaptation process. These findings provide novel insight into the cold-resistance mechanism in L. plantarum with potential application in low temperature fermented or preserved foods.

키워드

Proteomics; iTRAQ; Lactobacillus plantarum K25; cold-tolerance
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