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Comparative Whole Cell Proteomics of Listeria monocytogenes at Different Growth Temperatures

Journal of Microbiology and Biotechnology 2020년 30권 2호 p.259 ~ 270
 ( Won So-Yoon ) - Chungnam National University Department of Food and Nutrition

 ( Lee Jeong-Min ) - Chungnam National University Department of Food and Nutrition
 ( Kim Ji-Eun ) - Chungnam National University Department of Food and Nutrition
최형석 ( Choi Hyung-Seok ) - Chungnam National University Department of Food and Nutrition
김재한 ( Kim Jae-Han ) - Chungnam National University Department of Food and Nutrition

Abstract


Listeria monocytogenes is a gram-positive, facultative anaerobe food pathogen responsible for the listeriosis that mostly occurs during the low-temperature storage of a cold cut or dairy products. To understand the systemic response to a wide range of growth temperatures, L. monocytogenes were cultivated at a different temperature from 10oC to 42oC, then whole cell proteomic analysis has been performed both exponential and stationary cells. The specific growth rate increased proportionally with the increase in growth temperature. The maximum growth rate was observed at 37oC and was maintained at 42oC. Global protein expression profiles mainly depended on the growth temperatures showing similar clusters between exponential and stationary phases. Expressed proteins were categorized by their belonging metabolic systems and then, evaluated the change of expression level in regard to the growth temperature and stages. DnaK, GroEL, GroES, GrpE, and CspB, which were the heat&cold shock response proteins, increased their expression with increasing the growth temperatures. In particular, GroES and CspB were expressed more than 100-fold than at low temperatures during the exponential phase. Meanwhile, CspL, another cold shock protein, overexpressed at a low temperature then exponentially decreased its expression to 65-folds. Chemotaxis protein CheV and flagella proteins were highly expressed at low temperatures and stationary phases. Housekeeping proteins maintained their expression levels constant regardless of growth temperature or growth phases. Most of the growth related proteins, which include central carbon catabolic enzymes, were highly expressed at 30oC then decreased sharply at high growth temperatures.

키워드

Listeria monocytogenes; bacterial cell growth; temperature; protein expression; shotgun proteomics; bacterial proteomics
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