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Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake

Journal of Microbiology and Biotechnology 2020년 30권 2호 p.296 ~ 305
 ( Lee Nan-Yeong ) - Kyungpook National University School of Life Science and Biotechnology

최두호 ( Choi Doo-Ho ) - Kyungpook National University School of Life Science and Biotechnology
김미경 ( Kim Mi-Gyeong ) - Kyungpook National University School of Life Science and Biotechnology
정민지 ( Jeong Min-Ji ) - Kyungpook National University School of Life Science and Biotechnology
권해준 ( Kwon Hae-Jun ) - Kyungpook National University School of Life Science and Biotechnology
김동현 ( Kim Dong-Hyun ) - Kyungpook National University School of Life Science and Biotechnology
김영국 ( Kim Young-Guk ) - Kyungpook National University School of Life Science and Biotechnology
 ( di Luccio Eric ) - Kyungpook National University College of Natural Sciences School of Life Sciences
 ( Arioka Manabu ) - University of Tokyo Department of Biotechnology
윤혁준 ( Yoon Hyeok-Jun ) - Kyungpook National University School of Life Science and Biotechnology
김종국 ( Kim Jong-Guk ) - Kyungpook National University School of Life Science and Biotechnology

Abstract


Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.

키워드

(R)-(-)-1-octen-3-ol; Tricholoma matsutake; lipoxygenase; hydroperoxide lyase; Saccharomyces cerevisiae
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