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Roles of Protein Histidine Phosphatase 1 (PHPT1) in Brown Adipocyte Differentiation

Journal of Microbiology and Biotechnology 2020년 30권 2호 p.306 ~ 312
 ( Kang Joo-Ae ) - Korea Research Institute of Bioscience and Biotechnology Metabolic Regulation Research Center

강현섭 ( Kang Hyun-Sup ) - Korea Research Institute of Bioscience and Biotechnology Metabolic Regulation Research Center
배광희 ( Bae Kwang-Hee ) - Korea Research Institute of Bioscience and Biotechnology Metabolic Regulation Research Center
이상철 ( Lee Sang-Chul ) - Korea Research Institute of Bioscience and Biotechnology Metabolic Regulation Research Center
오경진 ( Oh Kyoung-Jin ) - Korea Research Institute of Bioscience and Biotechnology Metabolic Regulation Research Center
김원곤 ( Kim Won-Kon ) - Korea Research Institute of Bioscience and Biotechnology Metabolic Regulation Research Center

Abstract


Despite the importance of brown adipocytes as a therapeutic target for the prevention and treatment of obesity, the molecular mechanism underlying brown adipocyte differentiation is not fully understood. In particular, the role of post-translational modifications in brown adipocyte differentiation has not been extensively studied. Histidine phosphorylation is increasingly recognized an important process for protein post-translational modifications. In this study, we show that histidine phosphorylation patterns change during brown adipocyte differentiation. In addition, the expression level of protein histidine phosphatase 1 (PHPT1), a major mammalian phosphohistidine phosphatase, is reduced rapidly at the early phase of differentiation and recovers at the later phase. During white adipocyte differentiation of 3T3- L1 preadipocytes, however, the expression level of PHPT1 do not significantly change. Knockdown of PHPT1 promotes brown adipocyte differentiation, whereas ectopic expression of PHPT1 suppresses brown adipocyte differentiation. These results collectively suggest that histidine phosphorylation is closely linked to brown adipocyte differentiation and could be a therapeutic target for obesity and related metabolic diseases.

키워드

Brown adipogenesis; histidine phosphorylation; obesity; PHPT1
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