medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

A Stable hnRNA Species Confined in HeLa Cell Nucleus

Molecules and Cells 1990년 1권 1호 p.25 ~ 29
 ( Kim Jong-Hwa ) - Korea Advanced Institute of Science and Technology Department of Biological Science and Engineering

 ( Choi Yang-Do ) - Korea Advanced Institute of Science and Technology Department of Biological Science and Engineering
 ( Byun Si-Myung ) - Korea Advanced Institute of Science and Technology Department of Biological Science and Engineering

Abstract


To study the structure and physiological importances of the nucleus confined hnRNA, cDNA clones for relatively stable and abundant hnRNA have been isolated. HeLa cell hnRNA was purified by immunoaffinity chromatography using monoclonal antibody, 4F4, against the hnRNP proteins. First strand cDNA was synthesized using random hexamer as a primer and a eDNA library was constructed in λgtll vector. Recombinant phage clones of the hnRNA were isolated from the cDNA library by plaque hybridization with radioactively labeled first strand eDNA of the hnRNA as a probe. One of the positive clones, W16W, hybridizes to the discrete species of 1200-1300 bp nuclear non- olyadenylated hnRNA. Insert size of the cDNA clone was 809 bp. According to the Southern blotting analysis the hnRNA transcript is encoded by a single copy gene. Pulse-chase experiment in the presence of actinomycin D reveals that WI6W gene is transcribed by RNA polymerase II but the hnRNA transcript is relatively stable and quite abundant.

키워드

원문 및 링크아웃 정보
등재저널 정보
SCI(E)
MEDLINE
KCI