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DNA Helical Periodicity Imaged by Scanning Tunneling Microscopy

Molecules and Cells 1990년 1권 1호 p.57 ~ 59
 ( Kim Byung-Dong ) - Seoul National University Department of Horticulture

 ( Park Chan ) - Seoul National University Department of Horticulture
 ( Jeon Il-Cheol ) - Seoul National University Department of Horticulture

Abstract


pBR322 DNA molecules were deposited on highly oriented pyrolytic graphite and imaged in air vlith the scanning tunneling microscope. Clear view pf the heJicity of the uncoJited DNA was evident The pitch periodicity raJ1ged froIlJ 38 Å to 47 Å, and from 42 Å to 50 Å, and the diameter ranged from 20 Å to 25 Å Minor as well as major grooves were distinct from the vertical displacement proftle. A three dimensional image clearly revealed the side view of the protruding sugar phosphate backbones. A transmission electron microscope picture of pBR322 DNA is also presented to compare the quality of images and the scales of viewing area.

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