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RAD4 Gene Required for Nucleotide Excision Repair Is Not UV Inducible and Not Essential for Cell Viability in Saccharomyces cerevisiae

Molecules and Cells 1990년 1권 1호 p.87 ~ 92
 ( Kim Jae-Bum ) - Seoul National University Department of Zoology

 ( Choi In-Soon ) - Seoul National University Department of Zoology
 ( Kim Dong-Sun ) - Seoul National University Department of Zoology
 ( PARK SANG-DAI ) - Seoul National University Department of Zoology

Abstract


The RAD4 gene of Saccharomyces cerevisiae is essential for the incisioin step of UV-induced excision repair. We have determined the functional role and the inducibility of this gene. The 1.2-kb Pvull DNA fragment was internally disrupted from the cloned RAD4 gene (pPC1), and the residual fragment was ligated to produce the integrating plasmid pPC130. Wild type haploids transformed with this pPC130 showed UV-sensitivity, suggesting that the disrupted RAD4 sequence retains the complementing activity for rad4 mutation. Sporulation of diploids transformed with the UV-sensitive integrants gave rise to four viable haploids per tetrad, indicating that the RAD4 gene is not essential for the viability of haploids under nom1al growth conditions. The amount of RAD4 gene transcript was not increased when wild type cells were exposed to UV light Moreover, no induction of β-galactosidase activity was observed at various times after UV irradiation in yeast cells containing the RAD4-lacZ fusion, indicating that the RAD4 gene is not UV inducible.

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