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Biosynthesis of Plasmid-borne M1 RNA Transcripts and Their Effect on the Escherichia coli mpB Gene Expression

Molecules and Cells 1990년 1권 1호 p.103 ~ 109
 ( Kim Hi-Tae ) - Korea Advanced Institute of Science and Technology Department of Chemistry

 ( Jean Eun-Soon ) - Korea Advanced Institute of Science and Technology Department of Chemistry
 ( Jung Young-Hwan ) - Korea Advanced Institute of Science and Technology Department of Chemistry
 ( Yoon Kyung-Ran ) - Korea Advanced Institute of Science and Technology Department of Chemistry
 ( Lim Jeong-Hwa ) - Korea Advanced Institute of Science and Technology Department of Chemistry
 ( Cho Myung-Sun ) - Korea Advanced Institute of Science and Technology Department of Chemistry
 ( Park Chung-Ung ) - Korea Advanced Institute of Science and Technology Department of Chemistry
 ( Lee Young-Hoon ) - Korea Advanced Institute of Science and Technology Department of Chemistry

Abstract


M1 RNA transcripts synthesized from plasmids were analyzed and their effect on the Escherichia coli rnpB gene expression was examined to see whether the overexpressed M1 RNA transcripts affect rnpB promoter activity. Whereas abnormal M1 RNA transcripts were metabolically unstable, normal M1 RNA transcripts were accumulated. The accumulated M1 RNA transcripts showed no significant effect on the galactokinase synthesis directed by the rnpB promoter. This result suggests that M1 RNA itself might not be involved in the regulation of the rnpB gene expression for RNase P biosynthesis.

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