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Knockdown of Pyruvate Kinase M Inhibits Cell Growth and Migration by Reducing NF-kB Activity in Triple-Negative Breast Cancer Cells

Molecules and Cells 2019년 42권 9호 p.628 ~ 636
 ( Ma Chaobing ) - Zhengzhou University Affiliated Cancer Hospital Department of Breast Surgery

 ( Zu Xueyin ) - China-US (Henan) Hormel Cancer Institute
 ( Liu Kangdong ) - China-US (Henan) Hormel Cancer Institute
 ( Bode Ann M. ) - University of Minnesota Hormel Institute
 ( Dong Zigang ) - University of Minnesota Hormel Institute
 ( Liu Zhenzhen ) - Zhengzhou University Affiliated Cancer Hospital Department of Breast Surgery
 ( Kim Dong-Joon ) - China-US (Henan) Hormel Cancer Institute

Abstract


Altered genetic features in cancer cells lead to a high rate of aerobic glycolysis and metabolic reprogramming that is essential for increased cancer cell viability and rapid proliferation. Pyruvate kinase muscle (PKM) is a rate-limiting enzyme in the final step of glycolysis. Herein, we report that PKM is a potential therapeutic target in triple-negative breast cancer (TNBC) cells. We found that PKM1 or PKM2 is highly expressed in TNBC tissues or cells. Knockdown of PKM significantly suppressed cell proliferation and migration, and strongly reduced S phase and induced G2 phase cell cycle arrest by reducing phosphorylation of the CDC2 protein in TNBC cells. Additionally, knockdown of PKM significantly suppressed NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) activity by reducing the phosphorylation of p65 at serine 536, and also decreased the expression of NF-kB target genes. Taken together, PKM is a potential target that may have therapeutic implications for TNBC cells.

키워드

cell cycle; NF-kB; PKM1; PKM2; triple-negative breast cancer cells
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