잠시만 기다려 주세요. 로딩중입니다.

Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEFOprN Efflux Pump in Pseudomonas aeruginosa

Molecules and Cells 2019년 42권 12호 p.850 ~ 857
김수현 ( Kim Su-Hyeon ) - Seoul National University Department of Agricultural Biotechnology

김송희 ( Kim Song-Hee H. ) - Seoul National University Institute of Molecular Biology and Genetics
안진숙 ( Ahn Jin-Sook ) - Seoul National University Department of Agricultural Biotechnology
 ( Jo In-Seong ) - Seoul National University Department of Agricultural Biotechnology
 ( Lee Zee-Won ) - Seoul National University Department of Agricultural Biotechnology
최상호 ( Choi Sang-Ho ) - Seoul National University Department of Agricultural Biotechnology
하남출 ( Ha Nam-Chul ) - Seoul National University Department of Agricultural Biotechnology

Abstract


The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa, has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as a transcriptional activator of the MexEF-OprN efflux system. MexT consists of an N-terminal DNA-binding domain and a C-terminal regulatory domain (RD). Little is known regarding MexT ligands and its mechanism of activation. We elucidated the crystal structure of the MexT RD at 2.0 A resolution. The structure comprised two protomer chains in a dimeric arrangement. MexT possessed an arginine-rich region and a hydrophobic patch lined by a variable loop, both of which are putative ligand-binding sites. The three-dimensional structure of MexT provided clues to the interacting ligand structure. A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF-oprN promoter. Our findings enhance the understanding of the regulation of MexT-dependent activation of efflux pumps.

키워드

antibiotic resistance; crystal structure; lysR-type transcriptional regulator; MexT; Pseudomonas aeruginosa
원문 및 링크아웃 정보
 
등재저널 정보