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march5 Governs the Convergence and Extension Movement for Organization of the Telencephalon and Diencephalon in Zebrafish Embryos

Molecules and Cells 2020년 43권 1호 p.76 ~ 85
 ( Jung Jang-Ham ) - Chungnam National University Graduate School Department of Life Science

 ( Choi Issac ) - Chungnam National University Graduate School Department of Life Science
 ( Ro Hyun-Ju ) - Chungnam National University Graduate School Department of Life Science
 ( Huh Tae-Lin ) - Kyungpook National University College of Natural Sciences School of Life Sciences and Biotechnology
최준호 ( Choe Joon-Ho ) - Korea Advanced Institute of Science and Technology Department of Biological Sciences
 ( Rhee Myung-Chull ) - Chungnam National University Graduate School Department of Life Science

Abstract


MARCH5 is a RING finger E3 ligase involved in mitochondrial integrity, cellular protein homeostasis, and the regulation of mitochondrial fusion and fission. To determine the function of MARCH5 during development, we assessed transcript expression in zebrafish embryos. We found that march5 transcripts were of maternal origin and evenly distributed at the 1-cell stage, except for the mid-blastula transition, with expression predominantly in the developing central nervous system at later stages of embryogenesis. Overexpression of march5 impaired convergent extension movement during gastrulation, resulting in reduced patterning along the dorsoventral axis and alterations in the ventral cell types. Overexpression and knockdown of march5 disrupted the organization of the developing telencephalon and diencephalon. Lastly, we found that the transcription of march5 was tightly regulated by the transcriptional regulators CHOP, C/EBPα, Staf, Znf143a, and Znf76. These results demonstrate the essential role of March5 in the development of zebrafish embryos.

키워드

convergence and extension movement; diencephalon; March5/MITOL; telencephalon; ubiquitin proteasome system
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