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피부유래세포의 아포토시스 분석에 의한 췌장염 환자의 혈청 스크리닝

Screening of Sera from Patients with Pancreatitis by an Apoptosis Assay of Skin-derived Cells

대한소화기학회지 2019년 74권 4호 p.219 ~ 226
석애은 ( Seok Ae-Eun ) - 을지대학교 대학원 시니어헬스케어학과

손병관 ( Son Byoung-Kwan ) - 을지대학교 을지병원 소화기내과
이지영 ( Lee Ji-Young ) - 을지대학교 보건과학대학 임상병리학과
정광현 ( Chung Kwang-Hyun ) - 을지대학교 을지병원 소화기내과
이유림 ( Lee You-Rim ) - 을지대학교 대학원 시니어헬스케어학과
김두진 (  ) - 성남중앙병원 진단검사의학과
차병헌 ( Cha Byung-Hun ) - 을지대학교 보건과학대학 임상병리학과
강희규 ( Kang Hee-Gyoo ) - 을지대학교 보건과학대학 임상병리학과

Abstract


Background/Aims: An excessive inflammatory response is typical in acute pancreatitis and a significant cause of early mortality in severe acute pancreatitis. This is believed to be caused by inflammatory molecules or upregulated cytokine levels in the serum of patients. The aim of this study was to identify the serum-mediated apoptosis-inducing effects in acute pancreatitis patients.

Methods: A skin tissue-derived cell line, BJ, was treated for 24 hours with the sera of 22 healthy volunteers (control) and 71 acute pancreatitis patients (22 with gallstone pancreatitis, 16 with alcoholic pancreatitis, and 11 with pancreatitis with other causes) collected at the time of hospital admission (active) and discharge (resolved). Apoptosis was analyzed by flow cytometry.

Results: The average percentage of living cells, early apoptotic cells, and late apoptotic cells ranged from 78.8% to 85.0%, 5.5% to 7.3%, and 7.7% to 13.1%, respectively. The number of live cells increased significantly using the serum from the resolved state of gallstone-induced pancreatitis. In addition, the number of early apoptotic cells increased significantly using the serum from the resolved state of pancreatitis with other causes. The number of late apoptotic cells decreased significantly with the serum from the resolved state compared to the active state of gallstone- and alcohol-induced pancreatitis.

Conclusions: Serum samples from patients with pancreatitis induced a change in the apoptosis profiles of skin-derived cells. These results indicate changes in the serum components in patients with acute pancreatitis.

키워드

Acute pancreatitis; Skin tissue-derived cell line; Apoptosis; Alcohol-induced pancreatitis
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