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패혈증 치료제 개발을 위한 황백이 포함된 생약혼합제제 ABHC의 항균 효능

Antibacterial Activity of Herbal Complex ABHC for Development of Novel Therapeutic Agent Against Sepsis

생약학회지 2019년 50권 3호 p.191 ~ 197
이기만 ( Lee Ki-Man ) - 삼육대학교 약학과

이금선 ( Lee Geum-Seon ) - 삼육대학교 상담심리학과
김유리 ( Kim Yu-Ri ) - 삼육대학교 약학과
박준우 ( Park Jun-Woo ) - 삼육대학교 약학과
부경준 ( Boo Kyung-Jun ) - 삼육대학교 약학과
임동술 ( Yim Dong-Sool ) - 삼육대학교 약학과
강태진 ( Kang Tae-Jin ) - 삼육대학교 약학과

Abstract


Sepsis, an infectious disease, is a life-threatening condition that arises when the response to infection causes injury to tissues and organs. The purpose of this study was to demonstrate whether ABHC-1 and ABHC-2, two functional extracts from herbal complex, have an anti-bacterial effect against Escherchia coli in vivo, in vitro experimental model. ABHC-1 and ABHC-2 showed the antibacterial activity against the bacteria by paper disc method. The minimum inhibitory concentration (MIC) was measured using alamar blue reagent. The MIC was shown at 60 μg/ml from ABHC-1 and 500 μg/ml from ABHC-2 against E. coli. We next examined the effect of ABHCs on the production of inflammatory cytokine, such as tumor necrosis factor-a (TNF-a), which is related to the induction of inflammation, in RAW 264.7 cell. ABHC-1 and ABHC-2 increased TNF-a production of RAW 264.7 cell in a dose-dependent manner while two extract decreased TNF-a production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell in a dose-dependent manner. At a dose of 1 × 108 E. coli. i.p., non-treated mice were succumbed, while most of mice treated with ABHC-1 were survived. Therefore, our results suggest that ABHC-1 has anti-bacterial activity and can be a novel therapeutic agent against infectious diseases.

키워드

Herbal complex; Sepsis; Antibacterial activity; Tumor necrosis factor-a; Infection
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