잠시만 기다려 주세요. 로딩중입니다.

상황버섯의 예쁜꼬마선충 내의 항산화 효과

Antioxidative-activity of Phellinus baumii Pilat in Caenorhabditis elegans

생약학회지 2019년 50권 4호 p.299 ~ 304
김준형 ( Kim Jun-Hyeong ) - 우석대학교 약학대학

권강무 ( Kwon Kang-Mu ) - 우석대학교 약학대학
양재헌 ( Yang Jae-Heon ) - 아이나라
기별희 ( Ki Byeol-Hui ) - 아이나라
김대근 ( Kim Dae-Keun ) - 우석대학교 약학대학

Abstract


In order to research the anti-oxidative activity of methanol extract of Phellinus baumii Pilat (Hymenochaetaceae), Caenorhabditis elegans model system was used. Ethyl acetate soluble fraction of the methanol extract showed the most potent DPPH radical scavenging activity. The ethyl acetate fraction was measured on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance with reactive oxygen species (ROS) level in C. elegans. Furthermore, to see if regulation of stress-response gene is responsible for the increased stress tolerance of C. elegans which treated by the ethyl acetate fraction, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of P. baumii increased SOD and the catalase activities in a dose?dependent manner in C. elegans, reduced ROS accumulation dose-dependently. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the control worms.

키워드

Phellinus baumii Pilat; Caenorhabditis elegans; Anti-oxidative activity; Catalase; SOD
원문 및 링크아웃 정보
 
등재저널 정보