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Assessment of the Purity of Emodin by Quantitative Nuclear Magnetic Resonance Spectroscopy and Mass Balance

Natural Product Sciences 2019년 25권 3호 p.222 ~ 227
 ( Park So-Jung ) - Korea University College of Life Sciences and Biotechnology Department of Biosystems and Biotechnology

 ( Choi Yu-Jin ) - Korea University College of Life Sciences and Biotechnology Department of Biosystems and Biotechnology
 ( Do Giang Hoang ) - Korea University College of Life Sciences and Biotechnology Department of Biosystems and Biotechnology
 ( Seo Eun-Kyoung ) - Ewha Womans University College of Pharmacy Graduate School of Pharmaceutical Sciences
 ( Hyun Seung-hun ) - Korea University College of Life Sciences and Biotechnology Department of Environmental Science and Ecological Engineering
 ( Lee Dong-Ho ) - Korea University College of Life Sciences and Biotechnology Department of Biosystems and Biotechnology

Abstract


Quantitative nuclear magnetic resonance (qNMR) is a well-established method adopted by international pharmacopoeia for quantitative and purity analyses. Emodin is a type of anthraquinone, well known as the main active component of Fabaceae, Polygonaceae and Rhamnaceae. Purity analysis of emodin is usually performed by using the high-performance liquid chromatography (HPLC)-UV method. However, it cannot detect impurities such as salts, volatile matter, and trace elements. Using the qNMR method, it is possible to determine the compound content as well as the nature of the impurities. Several experimental parameters were optimized for the quantification, such as relaxation delay, spectral width, number of scans, temperature, pulse width, and acquisition time. The method was validated, and the results of the qNMR method were compared with those obtained by the HPLC and mass balance analysis methods. The qNMR method is specific, rapid, simple, and therefore, a valuable and reliable method for the purity analysis of emodin.

키워드

qNMR; Emodin; HPLC-UV; Mass balance
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