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Asiatic Acid Induces Apoptosis and Autophagy and Reduces MiR-17 and MiR-21 Expression in Pancreatic Cancer Cell Lines

Natural Product Sciences 2019년 25권 4호 p.298 ~ 303
 ( Jo Yoon-Gyung ) - Wonkwang University College of Pharmacy

김명재 ( Kim Myoung-Jae ) - Wonkwang University College of Pharmacy
신혜지 ( Shin Hye-Ji ) - Korea University College of Pharmacy
이기영 ( Lee Ki-Yong ) - Korea University College of Pharmacy
이은주 ( Lee Eun-Joo ) - Wonkwang University College of Pharmacy

Abstract


This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer.

키워드

Asiatic acid; Pancreatic cancer; Apoptosis; Autophagy; MicroRNA
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