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Development and Validation of HPLC-PDA Method and Pattern Recognition Analyses Using Eight Marker Compounds for the Quality Control Between the Seeds of Cuscuta chinensis Lam. and Cuscuta japonica Choisy

Natural Product Sciences 2019년 25권 4호 p.334 ~ 340
 ( Nguyen Duc Hung ) - Daegu Catholic University College of Pharmacy

 ( Zhao Bing Tian ) - Daegu Catholic University College of Pharmacy
 ( Le Duc Dat ) - Daegu Catholic University College of Pharmacy
마은숙 ( Ma Eun-Sook ) - Daegu Catholic University College of Pharmacy
민병순 ( Min Byung-Sun ) - Daegu Catholic University College of Pharmacy
우미희 ( Woo Mi-Hee ) - Daegu Catholic University College of Pharmacy

Abstract


Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-Ocaffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.

키워드

Cuscuta chinensis; Cuscuta japonica; Flavonoids; Caffeoylquinic acid; HPLC-PDA
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