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Soluble RAGE attenuates AngII-induced endothelial hyperpermeability by disrupting HMGB1-mediated crosstalk between AT1R and RAGE

Experimental & Molecular Medicine 2019년 51권 9호 p.113 ~ 113
 ( Jeong Ji-Su ) - Yonsei University Graduate Program in Science for Aging

 ( Lee Ji-Ye ) - Yonsei University College of Medicine Integrative Research Center for Cerebrovascular and Cardiovascular Diseases
 ( Lim Ju-Yeon ) - Yonsei University Graduate Program in Science for Aging
 ( Cho So-Young ) - Yonsei University Graduate Program in Science for Aging
안소영 ( An So-Young ) - Yonsei University College of Medicine Integrative Research Center for Cerebrovascular and Cardiovascular Diseases
 ( Lee Myung-Eun ) - Yonsei University College of Medicine Integrative Research Center for Cerebrovascular and Cardiovascular Diseases
 ( Yoon Na-Ra ) - Catholic University Incheon St. Mary’s Hospital Department of Pathology
 ( Seo Mi-Ran ) - Yonsei University College of Medicine Integrative Research Center for Cerebrovascular and Cardiovascular Diseases
 ( Lim So-Yeon ) - Catholic Kwandong University College of Medicine Institute for Bio-Medical Convergence
 ( Park Sung-Ha ) - Yonsei University College of Medicine Division of Cardiology

Abstract


Increased endothelial permeability, one of the earliest signs of endothelial dysfunction, is associated with the development of cardiovascular diseases such as hypertension and atherosclerosis. Recent studies suggest that the receptor for advanced glycation end products (RAGE) regulates endothelial permeability in inflammation. In the present study, we investigated the regulatory mechanism of RAGE in endothelial hyperpermeability induced by angiotensin II (Ang II), a well-known inflammatory mediator, and the potential therapeutic effect of soluble RAGE (sRAGE), a decoy receptor for RAGE ligands. For in vitro studies, Ang II-treated human umbilical vein endothelial cells (HUVECs) were treated with siRNA specific to either RAGE or sRAGE to disrupt RAGE-mediated signaling. Endothelial permeability was estimated using FITC-labeled dextran 40 and a resistance meter. To evaluate intercellular junction disruption, VE-cadherin expression was examined by western blotting and immunocytochemistry. Ang II increased the expression of the Ang II type 1 receptor (AT1R) and RAGE, and this increase was inhibited by sRAGE. sRAGE prevented Ang II-induced VE-cadherin disruption in HUVECs. For in vivo studies, Ang II-infused, atherosclerosis-prone apolipoprotein E knockout mice were utilized. Endothelial permeability was assessed by Evans blue staining of the aorta. Ang II increased endothelial barrier permeability, and this effect was significantly attenuated by sRAGE. Our data demonstrate that blockade of RAGE signaling using sRAGE attenuates Ang II-induced endothelial barrier permeability in vitro and in vivo and indicate the therapeutic potential of sRAGE in controlling vascular permeability under pathological conditions.

키워드

Senescence; Stress signalling
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