medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Interleukin-6 signaling regulates hematopoietic stem cell emergence

Experimental & Molecular Medicine 2019년 51권 10호 p.124 ~ 124
 ( Tie Ruxiu ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center

 ( Li Honghu ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center
 ( Cai Shuyang ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center
 ( Liang Zuyu ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center
 ( Shan Wei ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center
 ( Wang Binsheng ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center
 ( Tan Yamin ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center
 ( Zheng Weiyan ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center
 ( Huang He ) - Zhejiang University School of Medicine First Affiliated Hospital Bone Marrow Transplantation Center

Abstract


Hematopoietic stem cells (HSCs) produce all lineages of mature blood cells for the lifetime of an organism. In vertebrates, HSCs derive from the transition of the hemogenic endothelium (HE) in the floor of the embryonic dorsal aorta. Most recently, a series of proinflammatory factors, such as tumor necrosis factor-α, interferon-γ, and Toll-like receptor 4, have been confirmed to play a key role in HSC specification. However, the full complement of necessary signaling inputs remains unknown to date. Here, we show that interleukin-6R (IL6R) via IL6 is required and sufficient for HSC generation. We found that Notch activates IL6R by regulating its expression in the HE and in HSCs. The secretion of IL6 mainly originates from HSC-independent myeloid cells, but not from HSCs and their adjacent vascular endothelial cells. In addition, blocking IL6 signaling does not affect vascular development or the production of primitive erythrocytes. Taken together, our results uncover a previously obscure relationship between IL6 signaling and HSC production and provide new insights into HSC regeneration using proinflammatory factors in vitro.

키워드

Haematopoietic stem cells; Zebrafish
원문 및 링크아웃 정보
 
등재저널 정보