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Profiling of remote skeletal muscle gene changes resulting from stimulation of atopic dermatitis disease in NC/Nga mouse model

Korean Journal of Physiology & Pharmacology 2019년 23권 5호 p.367 ~ 379
 ( Lee Dong-Hee ) - Chung-Ang University College of Medicine Department of Physiology

 ( Seo Ye-Lim ) - Chung-Ang University College of Medicine Department of Physiology
 ( Kim Young-Won ) - Chung-Ang University College of Medicine Department of Physiology
 ( Kim Seong-Tae ) - Chung-Ang University College of Medicine Department of Physiology
 ( Choi Jeong-Yoon ) - Chung-Ang University College of Medicine Department of Physiology
 ( Moon Sung-Hee ) - Chung-Ang University College of Medicine Department of Physiology
 ( Bae Hye-mi ) - Chung-Ang University College of Medicine Department of Physiology
 ( Kim Hui-Sok ) - Chung-Ang University College of Medicine Department of Medicine
 ( Kim Han-Gyeol ) - Chung-Ang University College of Medicine Department of Medicine
 ( Kim Jae-Hyun ) - Chung-Ang University College of Medicine Department of Medicine
 ( Kim Tae-Young ) - Chung-Ang University College of Medicine Department of Medicine
 ( Kim Eun-Ho ) - Chung-Ang University College of Medicine Department of Medicine
 ( Yim Sue-Min ) - Chung-Ang University College of Medicine Department of Medicine
 ( Lim In-ja ) - Chung-Ang University College of Medicine Department of Physiology
 ( Bang Hyo-Weon ) - Chung-Ang University College of Medicine Department of Physiology

Abstract


Although atopic dermatitis (AD) is known to be a representative skin disorder, it also affects the systemic immune response. In a recent study, myoblasts were shown to be involved in the immune regulation, but the roles of muscle cells in AD are poorly understood. We aimed to identify the relationship between mitochondria and atopy by genome-wide analysis of skeletal muscles in mice. We induced AD-like symptoms using house dust mite (HDM) extract in NC/Nga mice. The transcriptional profiles of the untreated group and HDM-induced AD-like group were analyzed and compared using microarray, differentially expressed gene and functional pathway analyses, and protein interaction network construction. Our microarray analysis demonstrated that immune response-, calcium handling-, and mitochondrial metabolism-related genes were differentially expressed. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology pathway analyses, immune response pathways involved in cytokine interaction, nuclear factor-kappa B, and T-cell receptor signaling, calcium handling pathways, and mitochondria metabolism pathways involved in the citrate cycle were significantly upregulated. In protein interaction network analysis, chemokine family-, muscle contraction process-, and immune response-related genes were identified as hub genes with many interactions. In addition, mitochondrial pathways involved in calcium signaling, cardiac muscle contraction, tricarboxylic acid cycle, oxidation-reduction process, and calcium-mediated signaling were significantly stimulated in KEGG and Gene Ontology analyses. Our results provide a comprehensive understanding of the genome-wide transcriptional changes of HDM-induced AD-like symptoms and the indicated genes that could be used as AD clinical biomarkers.

키워드

Cytokines; Dermatitis; atopic; Microarray analysis; Mitochondria
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