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저산소성 조건에 노출된 구강암세포의 상피간엽전환관련 유전자 발현 프로파일

Epithelial-Mesenchymal Transition-Related Gene-Expression Profile in Hypoxic Oral Cancer Cells

대한구강악안면병리학회지 2019년 43권 6호 p.237 ~ 243
박대근 ( Park Dae-Geun ) - 부산대학교 치의학전문대학원 구경병리학교실

김소라 ( Kim So-Ra ) - 부산대학교 치의학전문대학원 구경병리학교실
우복희 ( Woo Vok-Hee ) - 부산대학교 치의학전문대학원 구강병리학교실
이지혜 ( Lee Ji-Hye ) - 부산대학교 치의학전문대학원 구경병리학교실
박혜련 ( Park Hae-Ryoun ) - 부산대학교 치의학전문대학원 구강병리학교실

Abstract


Hypoxia is one of the most common features of cancer. It is also associated with cancer progression and the acquisition of aggressiveness, which includes invasion and metastasis. Oral squamous cell carcinoma accounts for 90% of all oral cancers, and its 5-year survival rate is about 50%. Despite various attempts and trials, its prognosis has not improved. Among numerous adverse prognostic factors, hypoxia is suspected as one of the most important factors, as it increases the aggressiveness of oral cancer cells. We attempted to observe the effect of hypoxia on the expression of epithelial-mesenchymal transition markers in oral cancer cells. We analyzed and compared both the mRNA and protein expression levels of epithelial-mesenchymal markers using qRT-PCR and western blotting in both normoxic and hypoxic YD10B oral squamous cell carcinoma cells. Eighty-six genes were analyzed through real-time PCR using commercial microarray plates, performed in triplicate. Among the 86 genes, the expression of 24 were increased (≥ 2 fold) by hypoxia, while that of three genes was decreased (≥ 2 fold). Hypoxia significantly affects epithelial-mesenchymal transition-related genes. Further studies on the regulation of these genes may help to develop more efficient therapeutic modalities for oral cancer and to improve prognosis of oral cancer patients.

키워드

Hypoxia; Oral cancer; Epithelial-mesenchymal transition; TCF-4
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