잠시만 기다려 주세요. 로딩중입니다.

Comparison of inflammatory cytokine-inducing activity of lipopolysaccharides from major periodontal bacteria

International Journal of Oral Biology 2019년 44권 4호 p.160 ~ 164
김소희 ( Kim So-Hee ) - Chonnam National University School of Dentistry Department of Oral Microbiology

강인철 ( Kang In-Chol ) - Chonnam National University School of Dentistry Department of Oral Microbiology

Abstract


Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythia (Tf), Prevotella intermedia (Pi), and Fusobacterium nucleatum (Fn) are major periodontal pathogens. Lipopolysaccharides (LPSs) from periodontal bacteria play an important role in periodontal pathogenesis by stimulating host cells to produce inflammatory cytokines. In this study, highly pure LPSs from the five major periodontopathogens were prepared, and their monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α)-inducing activities were compared in human umbilical vein endothelial cells (HUVECs) and THP-1 macrophagic cells, respectively. In HUVECs, LPSs from Aa and Fn were potent stimulators for MCP-1 induction; however, LPSs from Pg, Pi, and Tf were much weaker MCP-1 inducers. In THP-1 cells, LPSs from Pg, Aa, and Fn were relatively strong inducers of TNF-α, whereas LPSs from Pi and Tf produced little activity. The Toll-like receptor (TLR)2/TLR4 dependency of various LPSs was also determined by measuring NF-κB reporter activity in TLR2- or TLR4-expressing 293 cells. LPSs from Aa, Fn, and Tf stimulated only TLR4; however, LPSs from Pg and Pi stimulated both TLR2 and TLR4. These results suggest that LPSs from major periodontal bacteria differ considerably in their cell-stimulating activity.

키워드

Periodontitis; Bacteria; Lipopolysaccharides; Cytokines; Toll-like receptors
원문 및 링크아웃 정보
등재저널 정보