medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

방풍류(防風類) 약재(藥材)의 신경세포보호효과 및 아세틸콜린에스터라제 저해 효과 비교

Comparative Study of Bang-poong (root of Saposhnikovia divaricata Schischkin)and Related Species on Neuroprotective and Acetylcholinesterase Inhibitory Effects

대한본초학회지 2019년 34권 5호 p.29 ~ 37
주인경 ( Ju In-Gyoung ) - 경희대학교 일반대학원 나노의약생명과학과

이승민 ( Lee Seung-Min ) - 경희대학교 약학대학 한약학과
최진규 ( Choi Jin-Gyu ) - 경희대학교 약학대학 한약학과
오명숙 ( Oh Myung-Sook ) - 경희대학교 일반대학원 나노의약생명과학과

Abstract


Objectives : Bang-poong (Saposhnikovia divaricata; SD) was traditionally used to treat inflammatory disorders. In this study, we aimed to investigate whether Bang-poong and related species including SD, Glehnia littoralis (GL), and Peucedanum japonicum (PJ) possess neuroprotective effects and acetylcholinesterase (AChE) inhibitory activities.

Methods : Roots of SD, GL and PJ were extracted with distilled water (DW) or 70% ethanol (EtOH). We assessed 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities of the extracts. To examine neuroprotective effects, we measured cell viability in PC12 or HT22 cells after treatment of the extracts with H2O2 or amyloid-beta (Aβ). To assess anti-neuroinflammatory effects, we measured the nitric oxide (NO) levels after treatment with the extracts and lipopolysaccharide (LPS) in BV2 microglial cells. In addition, we performed AChE inhibition assay to explore effects of the extracts on the cholinergic system.

Results : DW and EtOH extracts of SD, GL and PJ showed mild DPPH free radical scavenging activities. Also, DW extracts of GL and PJ showed protective effects against H2O2-induced toxicity in PC12 cells. In LPS-activated BV2 cells, EtOH extracts of SD, GL and PJ exerted inhibitory effects on NO production. Meanwhile, DW extracts of SD, GL and PJ inhibited the Aβ-induced cell death in HT22 cells. In addition, DW and EtOH extracts of GL exhibited remarkable inhibitory activities on AChE.

Conclusions : We demonstrated that SD, GL and PJ exert anti-oxidative, anti-neuroinflammatory and AChE inhibitory activities. These results indicate that SD, GL and PJ could be potential candidates for neurological disorders.

키워드

Saposhnikovia divaricata; Glehnia littoralis; Peucedanum japonicum; neuroprotection; neuroinflammation; acetylcholinesterase inhibition
원문 및 링크아웃 정보
 
등재저널 정보
KCI