잠시만 기다려 주세요. 로딩중입니다.

산수유(山茱萸) 메탄올 추출물이 편측 경동맥 폐색으로 유도된 생쥐의 허혈성 뇌손상에 미치는 영향

The protective effect of methanol extract of Corni Fructus on brain injury causedby unilateral common carotid artery occlusion in mice

대한본초학회지 2020년 35권 1호 p.1 ~ 8
최나리 ( Choi Na-Ri ) - 부산대학교 한의학전문대학원 한의학과

조성현 ( Jo Sung-Hyeon ) - 부산대학교 한의학전문대학원 한의학과
이세은 ( Lee Se-Eun ) - 부산대학교 한의과학연구소
이민지 ( Lee Min-Ji ) - 부산대학교 한의학전문대학원 한의과학과
조수인 ( Cho Su-In ) - 부산대학교 한의학전문대학원 한의학과

Abstract


Objectives:This study was conducted to evaluate the effects of Corni Fructus, the dried fruits of Cornus officinalis Sieb., on unilateral common carotid artery occlusion (UCCAO) in mouse model.

Methods:The Corni Fructus used in the experiment was extracted with anhydrous methanol, then filtered and freeze-dried. C57BL/6 mice used in the experiments were conducted left UCCAO surgery to set up UCCAO rodent model for mice. The mice were divided into five groups for evaluate the effect of methanol extract of Corni Fructus (COM) on UCCAO induced ischemic brain injury. The expression levels of nitric oxide in cerebrum and serum, body weight change were measured. To determine the effect of UCCAO and COM administration on brain neurons, morphological changes of the cerebrum through a microscope was conducted. And western blot was performed to confirm the underlying mechanism of neuroprotective effect of COM administration.

Results:COM administered UCCAO groups (CO50, CO150, and CO500) had no significant effects on nitric oxide production in ipsilateral hemisphere proteins and sera. The CO500, 500 mg/kg COM administration, attenuated UCCAO-induced p38 inflammatory signaling pathway and inflammatory mediators such as iNOS and COX-2. The CO500 group showed resilient morphological changes of hippocampus neuronal cells about brain damage caused by decreased flow of blood. These group also showed decreased inflammation and cellular stress response in neuronal cells.

Conclusions:From these results, COM has a neuroprotective property via moderating inflammatory factors and cellular stress inducing factors in brain cells.

키워드

Corni Fructus; brain injury; unilateral common carotid artery occlusion; neuroprotection
원문 및 링크아웃 정보
 
등재저널 정보
KCI