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MIA로 골관절염 유발된 동물모델에서 산수유 30% Ethanol 추출물의 개선 효과

Improvement Effect of Corni Fructus 30% Ethanol Extract by MIA-Induced Osteoarthritis Animal Model

대한본초학회지 2020년 35권 1호 p.35 ~ 44
김민주 ( Kim Min-Ju ) - 대구한의대학교 한의과대학 본초약리학교실

이진아 ( Lee Jin-A ) - 대구한의대학교 한의과대학 본초약리학교실
신미래 ( Shin Mi-Rae ) - 대구한의대학교 한의과대학 본초약리학교실
박해진 ( Park Hae-Jin ) - 대구한의대학교 산업보건연구소 DHU 바이오융복합시험센터
노성수 ( Roh Seong-Soo ) - 대구한의대학교 한의과대학 본초약리학교실

Abstract


Objectives:The objective of this study was to investigate the therapeutic effect of Corni Fructus 30% ethanol extract (CFE) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats.

Methods:The subjects were divided into 4 groups ; Normal group (N, n=10), MIA-induced osteoarthritis control group (Con, n=10), indomethacin 5 ㎎/㎏ treated group (INDO, n=10), CFE 200 ㎎/㎏ treated group (CFE, n=10). Blood and articulation tissues were collected after two weeks of drug administration. Oxidative stress was analyzed with reactive oxygen species (ROS), peroxynitrite (ONOO-). And the Nuclear factor erythroid-2 (Nrf2), heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, glutathione peroxidase-1/2 (GPx-1/2), Nuclear Factor Kappa B p65 (NF-Bp65), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF), interleukin-6 (IL-6), Interleukin 1 (IL-1), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were investigated by western blot.

Results:The administration of CFE showed a significant reduction of changes in relative hind paw weight distribution. Reactive oxygen species (ROS) and peroxy nitrite (ONOO-) levels of articulation tissues were significantly decreased in CFE compared to the control group. Western blot measurements of Nrf2, HO-1, SOD, catalase, GPx-1/2 showed that the CFE group was increased compared to the Con group. And western blot measurements of NF-Bp65, COX-2, iNOS, TNF, IL-6, IL-1 showed that the CFE group was reduced compared to the Con group. Also CFE group decreased MMP-1 and increased TIMP-1.

Conclusion:Based on the above results, it can be seen that osteoarthritis is improved when Corni Fructus 30% ethanol extract treated.

키워드

Corni Fructus; Osteoarthritis; monosodium iodoacetate (MIA); Oxidative stress; anti-oxidant; Inflammation; MMP; TIMP
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