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빈랑자와 천련자 복합물의 만성 역류성 식도염에서 보호 효과

The protective effect of Areca Semen and Toosendan Fructus mixture in a chronic model of reflux esophagitis

대한본초학회지 2020년 35권 1호 p.57 ~ 68
신미래 ( Shin Mi-Rae ) - 대구한의대학교 한의과대학 본초약리학교실

이진아 ( Lee Jin-A ) - 대구한의대학교 한의과대학 본초약리학교실
김민주 ( Kim Min-Ju ) - 대구한의대학교 한의과대학 본초약리학교실
안효진 ( An Hyo-Jin ) - 상지대학교 한의과대학 약리학교실
노성수 ( Roh Seong-Soo ) - 대구한의대학교 한의학과 본초약리학교실

Abstract


Objective:The aim of present study was to clarify the effect of Areca Semen and Toosendan Fructus Mixture (AT-mix) on chronic reflux esophagitis (CRE) in rats.

Methods:The antioxidant activity of AT-mix was measured through DPPH and ABTS radical scavenging activities in vitro. CRE was induced in SD rats (5 weeks, male) by ligating the border forestomach and granular portion with 2-0 silk and the duodenum near the pyloric portion was covered with 2-mm wide piece of 18-Fr Nelaton catheter. And then rats were treated AT-mix 200 mg/kg one daily for 14 days. The anti-oxidant and inflammatory protein levels were evaluated using western blotting.

Results:Gross lesion of esophageal mucosa after AT-mix treatment showed a superior enhancement compared with that of CRE control rats. AT-mix treatment strongly reduced both DPPH and ABTS radical scavenging activities (DPPH, IC50 8.15±0.14 g/mL; ABTS, IC50 24.69±0.03 g/mL, repspectively). Levels of the NADPH oxidase subunit including NOX4 and p22phox increased in CRE control rats. Otherwise, AT-mix treatment significantly reduced. The activation of Nuclear factor-erythroid 2-related factor 2 (Nrf2) led to significantly the up-regulation of HO-1. The inhibition of IB phosphorylation led to NF-B inactivation. Subsequently, NF-B inactivation significantly induced the decrease of COX-2, iNOS, TNF-, and IL-6 protein expressions.

Conclusion:Taken together, these results suggest that AT-mix treatment can attenuate the esophageal mucosal ulcer though inhibiting NF-B pathway and enhancing Nrf2/HO-1 pathway. Thus, the additional mechanism study about AT-mix would need for the development as a safe herbal therapy for CRE.

키워드

Areca Semen and Toosendan Fructus Mixture; chronic reflux esophagitis; esophageal mucosal ulcer; anti-oxidant; anti-inflammatory
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