잠시만 기다려 주세요. 로딩중입니다.

서과피(西瓜皮)의 전염증성 cytokine 억제효과

Exocarpium Citrulli suppresses pro-inflammatory cytokines

제한동의학술원논문집 2013년 11권 1호 p.57 ~ 66
손진원 ( Son Jin-Won ) - 대구한의대학교 한의과대학 외관과학교실

지선영 ( Jee Seon-Young ) - 대구한의대학교 한의과대학 외관과학교실
금수연 ( Keum Soo-Yeon ) - 대구한의대학교 한의과대학 외관과학교실

Abstract


Exocarpium Citrulli is the cortex of Fructus Citrulli. In Korean traditional medicine, it has been used to treat edema, dysuria, mouth and tongue sores. Although Exocarpium Citrulli has been known to possess anti-cancer activity, it has been rarely conducted to evaluate the anti-inflammatory activity. The present study was examined to evaluate the anti-inflammatory effects of the Exocarpium Gitrulli EtOH extracts (ECE) in vitro. Inflammatory mediators were detected after the addition of LPS with or without ECE in Raw 264.7 macrophage cell lines. LPS plays a key role in leading to the massive production of pro-inflammatory cytokines, such as TNF-a, 1L-1β and IL-6 in macrophages. In this study, cell viability assay (MTT assay) was conducted to determine the non-cytotoxic concentration of ECE. The dosages (0.1 and 0.3 mg/ml of ECE used in this study had no significant cytotoxicity during the experimental period. The relative level of NO was measured with Griess reagent. Pro-inflammatory cytokines were detected by EUSA assay. The cell was treated with 1 μg/ml of LPS 1 h before adding ECE. The level of NO and cytokines (TNF-a, IL-1β) in culture medium was increased by LPS application. However, these increases were attenuated by treatment with ECE.

키워드

원문 및 링크아웃 정보
등재저널 정보