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저장 기간에 따른 귤피(橘皮) Ethanol 추출물의 간 보호효과

Hepatoprotective effect of the CEE(ethanol extract of Citrus unshiu Markovich epicarp) depending on the storage period

제한동의학술원논문집 2016년 14권 1호 p.1 ~ 9
노규표 ( Noh Gyu-Pyo ) - 대구한의대학교 한의과대학 방제과학글로벌연구센터

조일제 ( Cho Il-Je ) - 대구한의대학교 한의과대학 방제과학글로벌연구센터
박정아 ( Park Chung-A ) - 대구한의대학교 한의과대학
김상찬 ( Kim Sang-Chan ) - 대구한의대학교 한의과대학 방제과학글로벌연구센터
변성희 ( Byun Sung-Hui ) - 대구한의대학교 한의과대학
박숙자 ( Park Sook-Ja ) - 대구한의대학교 한의과대학 방제과학글로벌연구센터

Abstract


Objectives : This study aimed to investigate the change of hepatoprotective activity depending on the storage period of CEE (ethanol extract of Citrus unshiu Markovich epicarp).

Methods : Citrus unshiu Markovich epicarp was washed, sliced and dried for 7 days at room temperature, and it was considered as zero month time. Citrus unshiu Markovich epicarp was further stored for 2, 4 and 6 months in the dry oven, which was set to 30℃. Hepatotoxicity was induced by exposing HepG2 cells to 100 μM cadmium for 3 hours. Cell viability was determined by MTT assay.

Results : CEE showed hepatoprotective effect better than water extract of Citrus unshiu Markovich epicarp in cadmium-treated HepG2 cells. Thus, we performed the cytoprotective efficacy experiment using CEE. Significant hepatoprotective activity was found in the all concentrations of CEE used in the experiment (10, 30, 100 ㎍/㎖). Depending on the prolonged storage period, CEE showed slightly weak hepatoprotective effect on average. However, there was no statistical significance in the change of hepatoprotective effect.

Conclusions : CEE showed dose-dependent hepatoprotective activity, and the significant difference in activity over storage time was not found. In conclusion, CEE can be used without change in activity, even if stored for 6 months at room temperature. In addition, the storage time of more than 6 months is recommended, to determine that the activity of Citrus unshiu Markovich epicarp is better depending on the storage period.

키워드

Ethanol extracts of Citrus unshiu Markovich epicarp (CEE); Cadmium; Hepatoprotective; Storage period
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