medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli

Journal of Microbiology 2019년 57권 10호 p.910 ~ 917
 ( Lee Jae-Jin ) - Chung-Ang University Department of Life Science

 ( Lee Dong-Ho ) - Chung-Ang University Department of Life Science
 ( Jeon Che-Ok ) - Chung-Ang University Department of Life Science
 ( Lee Kang-Seok ) - Chung-Ang University Department of Life Science

Abstract


Studies have shown that many enzymes involved in glycolysis are upregulated in Escherichia coli endoribonuclease G (rng) null mutants. However, the molecular mechanisms underlying the RNase G-associated regulation of glycolysis have not been characterized. Here, we show that RNase G cleaves the 5′ untranslated region of triosephosphate isomerase A (tpiA) mRNA, leading to destabilization of the mRNA in E. coli. Nucleotide substitutions within the RNase G cleavage site in the genome resulted in altered tpiA mRNA stability, indicating that RNase G activity influences tpiA mRNA abundance. In addition, we observed that tpiA expression was enhanced, whereas that of RNase G was decreased, in E. coli cells grown anaerobically. Our findings suggest that RNase G negatively regulates tpiA mRNA abundance in response to oxygen availability in E. coli.

키워드

glycolysis; mRNA abundance; rng; RNase G; tpiA
원문 및 링크아웃 정보
 
등재저널 정보