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다슬기(Semisulcospira libertine)의 미생물 오염도 평가 및해감 제거공정에 따른 저감화 효과

Investigation of Microbial Contamination in Semisulcospira libertine and Evaluation of Its Reduction Effects by Sediment Removal Treatment

한국식품위생안전성학회지 2019년 34권 4호 p.361 ~ 366
최만석 ( Choi Man-Seok ) - 경상대학교 해양산업연구소

전은비 ( Jun Eun-Bi ) - 경상대학교 해양산업연구소
최승호 ( Choi Seufng-Ho ) - 한국쓰리엠주식회사
방현조 ( Bang Hyun-Jo ) - 한국쓰리엠주식회사
박신영 ( Park Shin-Young ) - 경상대학교 해양산업연구소

Abstract


In this study, microbial contamivation semisulcospira libertine and effect of sedimentation treatment of major bacterial and fungal pathogens were investigated. The total aerobic bacteria, coliforms, Escherichia coli, Staphylococcus aureus, and yeast and mold present in raw and water-dipped Semisulcospira libertine were enumerated using the standard plate count methods on using the standard plate method on potato dextrose agar (PDA), 3M Petrifilm for coliforms / E. coli, 3M Petrifilm for S. aureus, and plate count agar (PCA), respectively. In analysis of microbial contamination of raw Semisulcospira libertine, the total aerobic bacteria, coliforms, and yeast and mold were monitored as 6.40, 2.70, and 6.79 log 10 CFU/g, respectively. Both E. coli and S. aureus were not detected (detection limit: 10 CFU/g). However, Semisulcospira libertine dipped in ground water for 3 hours had higher contamination levels of all natural indigenous microorganisms than raw Semisulcospira libertine. Especially, E. coli was detected as 2.46 log 10 CFU/g in the ground water-dipped Semisulcospira libertine. The total aerobic bacteria in the ground water-dipped Semisulcospira libertine was not significantly reduced (p>0.05) compared to that in the raw Semisulcospira libertine. Moreover, coliforms were significantly increased (p>0.05) in all water-dipped Semisulcospira libertine. Only fungi were slightly reduced (less than 0.2 log) (p>0.05) in the tap water-dipped Semisulcospira libertine by comparison with the raw Semisulcospira libertine. The results of this study suggest that the use of chemical sterilizing agents and other physical methods in the washing stage will be necessary for the microbial reduction in raw Semisulcospira libertine because the use of sediment removal treatment by ground or tap water did not affect the microbiological safety of the raw Semisulcospira libertine.

키워드

Semisulcospira libertine; Microbial contamination; Sediment removal treatment; Ground water; Tap water
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