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퇴비에서 식중독균 검출을 위한 DNA 추출 방법 비교

Comparison of DNA Extraction Methods for the Detection of Foodborne Pathogenic Bacteria from Livestock Manure Composts

한국식품위생안전성학회지 2019년 34권 6호 p.557 ~ 561
김성연 ( Kim Sung-Youn ) - 국립농산물품질관리원 시험연구소 안전성분석과

서동연 ( Seo Dong-Yeon ) - 국립농산물품질관리원 시험연구소 안전성분석과
문지영 ( Moon Ji-Young ) - 국립농산물품질관리원 시험연구소 안전성분석과

Abstract


This study investigated the efficacy of DNA extraction methods for real-time PCR detection of foodborne pathogenic bacteria in livestock manure composts. Livestock manure composts were inoculated with Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, Bacillus cereus and incubated in enrichment broth. For DNA extraction, enriched samples were treated following boiling method, by chloroform, C 18 powder, and proteinase K. As a result, 4 species of bacteria were detected by real-time PCR when subjected to boiling for 30 min and treated with proteinase K. These results suggest that detection of foodborne pathogens by real-time PCR from livestock manure composts could be applicable using effective DNA extraction methodology such as the boiling method or proteinase K.

키워드

Livestock manure composts; Real-time PCR; Boiling method; Chloroform; Proteinase K
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