medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Interaction between Brucella melitensis 16M and small ubiquitin-related modifier 1 and E2 conjugating enzyme 9 in mouse RAW264.7 macrophages

Journal of Veterinary Science 2019년 20권 5호 p.54 ~ 54
 ( Yi Jihai ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine

 ( Wang Yueli ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( Li Qifeng ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( Zhang Huan ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( Shao Zhiran ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( Deng Xiao Yu ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( He Jinke ) - Shihezi University School of Life Science Department of Biology
 ( Xiao Chencheng ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( Wang Zhen ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( Wang Yong ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine
 ( Chen Chuangfu ) - Shihezi University College of Animal Science and Technology Department of Veterinary Medicine

Abstract


Brucella is an intracellular pathogen that invades a host and settles in its immune cells; however, the mechanism of its intracellular survival is unclear. Modification of small ubiquitin-related modifier (SUMO) occurs in many cellular activities. E2 conjugating enzyme 9 (Ubc9) is the only reported ubiquitin-conjugating enzyme that links the SUMO molecule with a target protein. Brucella's intracellular survival mechanism has not been studied with respect to SUMO-related proteins and Ubc9. Therefore, to investigate the relationship between Brucella melitensis 16M and SUMO, we constructed plasmids and cells lines suitable for overexpression and knockdown of SUMO1 and Ubc9 genes. Brucella 16M activated SUMO1/Ubc9 expression in a time-dependent manner, and Brucella 16M intracellular survival was inhibited by SUMO1/Ubc9 overexpression and promoted by SUMO1/Ubc9 depletion. In macrophages, Brucella 16M-dependent apoptosis and immune factors were induced by SUMO1/Ubc9 overexpression and restricted by SUMO1/Ubc9 depletion. We noted no effect on the expressions of SUMO1 and Ubc9 in B. melitensis 16M lipopolysaccharide-prestimulated mouse RAW264.7 macrophages. Additionally, intracellular survival of the 16M△VirB2 mutant was lower than that of Brucella 16M (p < 0.05). VirB2 can affect expression levels of Ubc9, thereby increasing intracellular survival of Brucella in macrophages at the late stage of infection. Collectively, our results demonstrate that B. melitensis 16M may use the VirB IV secretion system of Brucella to interact with SUMO-related proteins during infection of host cells, which interferes with SUMO function and promotes pathogen survival in host cells.

키워드

Brucella; coupling enzyme Ubc9; interaction; intracellular; SUMO
원문 및 링크아웃 정보
   
등재저널 정보