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Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip

Journal of Veterinary Science 2020년 21권 1호 p.4 ~ 4
 ( Kim Jung-Min ) - Kyonggi University College of Fusion Science Department of Life Science

 ( Lim Su-Jin ) - Kyonggi University College of Fusion Science Department of Life Science
 ( Kim So-Min ) - Kyonggi University College of Fusion Science Department of Life Science
 ( Kim Moon-Jung ) - Kyonggi University College of Fusion Science Department of Life Science
 ( Kim Byoung-Hee ) - Kyonggi University College of Fusion Science Department of Life Science
 ( Tai Truong A. ) - Kyonggi University College of Fusion Science Department of Life Science
 ( Kim Seon-Mi ) - Kyonggi University College of Fusion Science Department of Life Science
윤병수 ( Yoon Byoung-Su ) - Kyonggi University College of Fusion Science Department of Life Science

Abstract


Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.

키워드

Ultra-rapid reverse transcription-qPCR; DNA-chip; deformed wing virus; field detection
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