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The Sterilization Effect of Methylene Blue, Formalin, and Iodine on Egg and Adult Stage of Marine Medaka, Oryzias dancena

발생과생식 2019년 23권 3호 p.199 ~ 211
 ( Park In-Seok ) - Korea Maritime and Ocean University College of Ocean Science and Technology Division of Marine Bioscience

백세원 ( Baek Se-Won ) - Korea Maritime and Ocean University College of Ocean Science and Technology Division of Marine Bioscience
문기환 ( Moon Ki-Hwan ) - Korea Maritime and Ocean University College of Ocean Science and Technology Division of Marine Bioscience

Abstract


The sterilization effects of methylene blue (MB), formalin, and iodine on the egg of marine medaka, Oryzias dancena, were investigated for disinfecting naididae worm, Chaetogaster diastrophus through sterilization. To determine harmfulness of MB, formalin, and iodine, lethal concentrations 50 (LC50) of three chemicals were analyzed in the eggs of ma?rine medaka. The sterilized periods of each chemical were set at 1 hr. Sterilized rates of naididae worm in each chemical were significantly affected and increased drastically as the concentration of each chemical increased (p<0.05). Sterilization abilities of naididae worm were most effective for formalin, but survival rates of egg and hatched rates for formalin were lowest among each chemical. The LC50 of MB over 96 hrs were 185.26, 103.84, and 127.15 ppm for adults, juveniles, and eggs re?spectively. The toxic effects of MB were clearly dose dependent for each life stage (p<0.05). The toxicity sensitivity of juve?niles to MB was dramatically higher than that of other groups. In 48 hrs after sterilization, cortisol and glucose concentrations of the adult group with MB treatment were significantly higher than those of the adult group with no treatment (p<0.05). This research provides useful data on sterilization effect of MB, formalin, and iodine, acute toxicity in marine medaka egg and tox?icity, sensitivity of life stage of MB in marine medaka.

키워드

Acute toxicity; Formalin; Iodine; Methylene blue; Oryzias dancena; Sterilization
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