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Dietary Exposure to Transgenic Rice Expressing the Spider Silk Protein Fibroin Reduces Blood Glucose Levels in Diabetic Mice: The Potential Role of Insulin Receptor Substrate-1 Phosphorylation in Adipocytes

발생과생식 2019년 23권 3호 p.223 ~ 229
 ( Park Ji-Eun ) - Dong-A University College of Medicine Department of Anatomy and Cell Biology

정연재 ( Jeong Yeon-Jae ) - Dong-A University College of Medicine Department of Anatomy and Cell Biology
박준범 ( Park Joon-Beom ) - Dong-A University College of Medicine Department of Anatomy and Cell Biology
김혜영 ( Kim Hye-Young ) - Dong-A University College of Medicine Department of Anatomy and Cell Biology
 ( Yoo Young-Hyun ) - Dong-A University College of Medicine Department of Anatomy and Cell Biology
이광식 ( Lee Kwang-Sik ) - Dong-A University College of Life Science and Natural Resources
양원태 ( Yang Won-Tae ) - Dong-A University College of Life Science and Natural Resources
 ( Kim Doh-Hoon ) - Dong-A University College of Life Science and Natural Resources
 ( Kim Jong-Min ) - Dong-A University College of Medicine Department of Anatomy and Cell Biology

Abstract


Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance (IR). T2DM is correlated with obesity and most T2DM medications have been developed for enhancing insulin sensitivity. Silk protein fibroin (SPF) from spiders has been suggested as an attractive biomaterial for medical purposes. We generated transgenic rice (TR) expressing SPF and fed it to diabetic BKS.Cg-m+/+Leprdb mice to monitor the changes in blood glucose levels and adipose tissue proteins associ?ated with energy metabolism and insulin signaling. In the present study, the adipocyte size in abdominal fat in TR-SPF-fed mice was remarkably smaller than that of the control. Whereas the adenosine monophosphate-activated protein kinase (AMPK)-activated protein kinase and insulin receptor substrate 1 (IRS1) protein levels were increased in abdominal adipose tissues after TR-SPF feeding, levels of six-transmembrane protein of prostate 2 (STAMP2) proteins decreased. Phosphoryla?tion of AMPK at threonine 172 and IRS1 at serine 307 and tyrosine 632 were both increased in adipose tissues from TR-SPF?fed mice. Increased expression and phosphorylation of IRS1 at both serine 307 and tyrosine 632 in adipose tissues indicated that adipocytes obtained from abdominal fat in TR-SPF-fed mice were more susceptible to insulin signaling than that of the control. STAMP2 protein levels decreased in adipose tissues from TR-SPF-fed mice, indicating that STAMP2 proteins were reducing adipocytes that were undergoing lipolysis. Taken together, this study showed that TR-SPF was effective in reducing blood glucose levels in diabetic mice and that concurrent lipolysis in abdominal adipocytes was associated with alterations of AMPK, IRS1, and STAMP2. Increased IRS1 expression and its phosphorylation by TR-SFP were considered to be particular?ly important in the induction of lipolysis in adipocytes, as well as in reducing blood glucose levels in this animal model.

키워드

Spider silk protein fibroin; Transgenic rice; IRS1; Adipocytes; Diabetic mouse
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