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화어전(化瘀煎)이 조골세포 및 경골골절 유발 생쥐의 골유합에 미치는 영향

Affirmative Effect of Hwaweo-jeon (Huayu-jian) in Osteoblast Cells and Tibia Fracture-induced Mice

한방재활의학과학회지 2020년 30권 1호 p.13 ~ 29
이수환 ( Lee Soo-Hwan ) - 상지대학교 일반대학원 한의학과

 ( Parichuk Kira ) - 상지대학교 한의과대학 약리학교실
차윤엽 ( Cha Yun-Yeop ) - 상지대학교 부속한방병원 한방재활의학과

Abstract


Objectives : This study was performed to decide the bone union effect of Hwaweo-jeon on tibia fractured mice.

Methods : In this study, laboratory experiments were implemented by the stage of in vitro and in vivo. In in vitro, MC3T3-E1 cells were treated with various concentration of Hwaweo-jeon extract (HWJ). To investigate effect of HWJ for osteoblast, relative mRNA expression of 5 substances (alkaline phosphatase [ALP], runt-related transcription factor 2 [Runx2], osteocalcin [OCN], osterix [OSX] and collagen type Ⅱ alpha 1 chain [Col2a1]) was used as a marker of osteogenesis. In order to determine HWJ’s effect for fracture healing, relative gene expression level of ALP, Runx2, OCN, OSX and Col2a1 were used to find out the influence to osteoblast. Furthermore, receptor activator of nuclear factor kappa-B ligand and osteoprotegerin relative mRNA expression were used to estimate the impact to osteoclast. Also, X-ray was used for the purpose of identifying bone union in tibia-fracture mouse model.

Results : In in vitro experiment, most part of relative mRNA expression were increased compared to control group. In in vivo and in vitro experiment, HWJ induced osteoblast activitation by verifying relative mRNA expression of 5 substances. And in vivo experiment, we can also identify that HWJ triggered osteoclast activation during early stage of tibia fracture. Furthermore, X-ray pictures show noticeable recovery of tibia fracture.

Conclusions : HWJ extract promotes bone union by facilitating the osteoblast. But, HWJ may occur liver & kidney toxicity over specific concentration. Therefore, when HWJ is applied to human body, doctors have to follow up the liver function test & renal function test of patient.

키워드

Hwaweo-jeon (Huayu-jian); Tibia fractured mice; Osteoblasts; Osteoclasts; Bone union; mRNA expression
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