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창상을 유발한 흰쥐에서 金銀花 추출물의 치료 효과

The Effect of Lonicera japonica Extract in Wound-induced Rats

한방재활의학과학회지 2020년 30권 1호 p.47 ~ 61
원제훈 ( Won Je-Hoon ) - 동국대학교 경주한의원 한방재활의학과

우창훈 ( Woo Chang-Hoon ) - 대구한의대학교 한의과대학 한방재활의학과교실

Abstract


Objectives :This study is carried out to investigate the effects of Lonicera japonica in wound-induced rats.

Methods :Rats were divided into 5 groups; normal (Nor), control (Veh), positive comparison (PC), Lonicera japonica 100 mg/kg (LL), Lonicera japonica 200 mg/kg (LH), each n=8. Total polyphenol and flavonoid were quantified. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3 ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging activation were measured. Reactive oxygen species (ROS) was measured in serum. Antioxidant factors and inflammatory factors were measured in skin tissue, and also hydroxyproline content. Skin tissue was analyzed by Hematoxylin & Eosin and Masson’s trichrome staining method.

Results :Total polyphenol and flavonoid were 32.86±0.14 mg/g and 67.17±0.57 mg/g. The IC50 values of DPPH and ABTS free radical scavenging activation were 26.69±1.50 μg/mL and 49.33±4.52 μg/mL. ROS was significantly lower in LL and LH groups. Nuclear factor-erythroid 2-related factor 2 (Nrf2) was significantly higher in LH group and higher in LL group but not significant. Superoxide dismutase 1 (SOD-1), catalase, and heme oxygenase 1 (HO-1) were significantly higher in LL and LH groups. Nuclear factor kappa-B p65 (NF-κBp65), phosphorylated iκBα (p-iκBα), cyclooxygenase 2 (COX-2), and tumor necrosis factor alpha (TNF-α) were significantly lower in LL and LH groups. Hydroxyproline was significantly higher in LL and LH groups. The histopathologic analysis showed that skin tissue had recovered further more in LL and LH groups than in Veh group.

Conclusions :These results suggest that Lonicera japonica has the anti-oxidant, anti-inflammatory and healing effects in wound-induced rats.

키워드

Lonicera japonica; Antioxidants; Inflammation; Wound healing
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