잠시만 기다려 주세요. 로딩중입니다.

Identification of Candida Species Using 26S Ribosomal RNA Gene Sequencing in Patients with Periodontitis

Journal of Bacteriology and Virology 2019년 49권 4호 p.212 ~ 220
 ( Al-Dabbagh Nebras Nasir ) - University of Babylon College of Dentistry Department of Microbiology

 ( Al-Janabi Wissam Hamid ) - University of Babylon College of Dentistry Department of Microbiology
 ( Al-Shuhaib Mohammed Baqur Sahib ) - Al-Qasim Green University College of Agriculture Department of Animal Production

Abstract


The infection with Candida spp. for oral cavity is being increasingly reported. However, its variations have not yet been specifically described in periodontitis. The present study was conducted to use an uniplex 26S rRNA-based amplicons to detect and discriminate Candida using only one pair of ribosomal primers. A total of 50 patients with chronic periodontitis was involved in the study. Pure Candida colonies were isolated from 23 patients and genomic DNA was extracted, and PCR was conducted. Direct DNA sequencing followed by comprehensive phylogenetic analyses were performed to confirm the identity of Candida colonies. Results indicated that the ration of Candida-infected patients was 46%, with a high prevalence of C. albicans, followed by remarkably lower ratios of C. parapsilosis, C. glabrata, C. kefyr, and C. dubliniensis respectively. Phylogenetic analyses indicated obvious discrimination amongst the analyzed Candida species as each observed species occupied a distinctive phylogenetic position. The current results reported a simple, efficient, and low-cost detection of five species of Candida without the need for other costly techniques of molecular screening. The current findings may help dentists to easily take a snapshot of the patterns of Candida infection in periodontitis cases to assess the nature and grade of infection.

키워드

26S ribosomal RNA; Candida; infection; DNA sequence analysis; Oral cavity
원문 및 링크아웃 정보
 
등재저널 정보