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Comparison of Red Blood Cell, White Blood Cell and Differential Counts between UF-5000 System and Manual Method

임상검사와정도관리 2019년 41권 3호 p.172 ~ 178
구모세 ( Koo Mo-Sae ) - Chungnam National University College of Medicine Department of Laboratory Medicine

 ( Lim Jin-Sook ) - Chungnam National University College of Medicine Department of Laboratory Medicine
 ( Kim Seon-Young ) - Chungnam National University College of Medicine Department of Laboratory Medicine
 ( Koo Sun-Hoe ) - Chungnam National University College of Medicine Department of Laboratory Medicine
 ( Kwon Gye-Cheol ) - Chungnam National University College of Medicine Department of Laboratory Medicine

Abstract


Background: Analysis of body fluids provides important information for assessing various medical conditions. We aimed to validate the analytical and diagnostic performance of the Sysmex UF-5000 (Sysmex, Japan) system for the analysis of different body fluids.

Methods: Eighty body fluid samples were analyzed using the UF-5000 system in the body fluid mode and light microscopy. Body fluids included ascitic, pleural, and cerebrospinal fluid (CSF), as well as other fluid samples.

Results: A comparison between the UF-5000 system and manual counting demonstrated good correlations with regard to red (r =0.6555) and white blood cell (r =0.9666) counts. The UF-5000 system also demonstrated good performance for differential cell counting (r =0.9028). CSF particularly showed a good correlation.

Conclusions: The use of the UF-5000 system for cell counting and differential analysis of body fluid samples might be an effective and automated alternative to chamber counting in laboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness.

키워드

Sysmex UF-5000; Automation; Laboratory; Body fluids
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