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Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR

Food Science and Biotechnology 2019년 28권 5호 p.1593 ~ 1598
 ( Suh Seung-Man ) - Kyung Hee University Department of Food Science and Biotechnology

 ( Park Saet-Byul ) - Kyung Hee University Department of Food Science and Biotechnology
 ( Kim Mi-Ju ) - Kyung Hee University Department of Food Science and Biotechnology
김해영 ( Kim Hae-Yeong ) - Kyung Hee University Department of Food Science and Biotechnology

Abstract


Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes Cyclophilin (tomato), Mdtl 1 (apple), Pru p 2.01A (peach) and Pectin methylesterase inhibitor (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.

키워드

Food allergy; Fruit allergen; Multiplex PCR; Peach; Tomato; Apple; Kiwi
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