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저병원성 조류인플루엔자와 뉴캐슬 바이러스의 복합감염에 따른 닭 림프조직 병변의 특성 및 바이러스 검출

Pathology and virus distribution in the lymphoid tissues of chicks co-infection with H9N2 Avian influenza and Newcastle disease virus

한국가축위생학회지 2019년 42권 3호 p.135 ~ 144
이성민 ( Le Sung-Min ) - 충남대학교 수의과대학

조은상 (  ) - 산업안전보건공단 산업안전보건연구원
최환원 ( Choi Hwan-Won ) - 중앙백신연구소
최보현 ( Choi Bo-Hyun ) - 수가축병원
손화영 ( Son Hwa-Young ) - 충남대학교 수의과대학


Low pathogenic avian influenza (LPAI; H9N2) and Newcastle disease (ND) are economically important poultry diseases in Korea. In this study, we investigated pathological features and virus distribution in the lymphoid tissues of chicks experimentally infected with H9N2 and/or ND virus. Six-weeks-old SPF chickens were divided into 4 groups, Control (C), H9N2 (E1), NDV (E2), and H9N2+NDV (E3). E1 group was challenged with 0.1 ml A/Kr/Ck/01310/01 (H9N2) 105.6 EID50 intranasally, E2 group was challenged with 0.5 ml KJW (NDV) 105.0∼106.0 ELD50 intramuscularly, and E3 group was challenged with H9N2, followed 7 days later by NDV. In histopathological examination, E1 group showed depletion and necrosis in bursa of Fabricius, thymus, cecal tonsil, and spleen, whereas E2 and E3 groups were noted severe lymphocyte depletion and necrosis with destruction of lymphoid organs structures. In TUNEL assay, apoptotic bodies were detected in lymphoid organs of all experimental groups, which was most severe in E3 group. H9N2 and ND viruses were predominantly detected in cecal tonsil of E1, E2, and E3 groups by PCR and immunohistochemistry (ICH). In conclusion, co-infection of H9N2 with NDV caused severe pathologic lesions and apoptosis in lymphoid tissues compared to single infections.


SPF chicken; LPAI; ND; Lymphoid tissues; Pathology
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