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닭 도축장에서 분리한 nalidixic acid 내성 Salmonella 균의 gyrA 유전자 돌연변이

Mutation in gyrA gene of nalidixic acid-resistant Salmonella isolates isolated from poultry slaughterhouse

한국가축위생학회지 2019년 42권 3호 p.153 ~ 159
조재근 ( Cho Jae-Keun ) - 대구광역시 보건환경연구원

손규희 ( Sohn Kyu-Hee ) - 경북대학교 수의과대학
김경희 ( Kim Kyung-Hee ) - 대구광역시 보건환경연구원
김정미 ( Kim Jeong-Mi ) - 대구광역시 보건환경연구원
박대현 ( Park Dae-Hyun ) - 대구광역시 보건환경연구원
이정우 ( Lee Jung-Woo ) - 대구광역시 보건환경연구원

Abstract


The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6’)-lb-cr and qepA in 40 nalidixic acid- resistant (NAR) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 NAR Salmonella isolates was 128∼512 μg/mL and <0.125∼0.25 μg/mL, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All NAR Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: Asp87→glycine, Ser83→tyrosine. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.

키워드

Salmonella; gyrA; Nalidixic acid; Ciprofloxacin; PMQR
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