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Bacillus polymyxa YU-101 균이 생산하는 생물고분자의 이화학적 특성

Physicochemical Property of Biopolymer Produced from Bacillus polymyxa YU-101

산업식품공학 2019년 23권 3호 p.178 ~ 185
장재권 ( Jang Jae-Kweon ) - 청강문화산업대학교 푸드스쿨 식품영양전공

Abstract


For the studies of production and availability of biopolymer from Bacillus polymyxa YU-101, its physicochemical properties were investigated. Crude biopolymer, precipitated by isopropyl alcohol from culture broth, was fractionated as two fraction of PSI and PSII by ion chromatography on DEAE-cellulose. PSII accounts for 95% of the total crude biopolymer and was an acidic biopolymer with 17.5% uronic acid. The molecules weight of the acidic biopolymer (PSII) by gel chromatography was to be approximately estimated 9.65×105 Da. As a result of cetylpyridinium chloride (CPC) treatment to determine the critical point of the salt for separation and purification of acidic biopolymer (PSII), the critical concentration of salt (NaCl and CaCl2) were 0.4-0.5 M and glucose, mannose and galactose were the major constituents. A crude biopolymer neutralization equivalent of 372 was also obtained, assuming that the crude biopolymer is a polymer of six carbon sugars with an average molecular weight of 162, suggesting that there is one anion per about 2.3 residues. Smith degradation of crude biopolymer resulted in the formation of glucose, glycerol, and erythritol as the final redox materials, and it was assumed to be a biopolymer composed of 1,4, and 1,6 bond linkage containing side chains.

키워드

Bacillus polymyxa YU-101; biopolymer; acidic biopolymer; neutral biopolymer
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