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In vitro estimation of metal-induced disturbance in chicken gut-oviduct chemokine circuit

Molecular & Cellular Toxicology 2019년 15권 4호 p.443 ~ 452
 ( Kim Ki-Hyung ) - Pusan National University School of Medicine Department of Obstetrics and Gynecology

 ( Kim Ju-Il ) - Pusan National University Biomedical Research Institute Department of Biomedical Sciences
 ( Han Jae-Yong ) - Seoul National University Department of Agricultural Biotechnology
 ( Moon Yu-Seok ) - Pusan National University Biomedical Research Institute Department of Biomedical Sciences


Backgrounds: Heavy metals affect various processes in the embryonic development. Embryonic fibroblasts (EFs) play key roles in the innate recognition and wound healing in reproductive tissues.

Methods: Based on the relative toxicities of different inorganic metals and inorganic nonmetallic compounds against murine and chicken EF cells, mechanistic estimations were performed based on transcriptomic analyses.

Results: Lead (II) acetate induced preferential injuries in the chicken EF and mechanistic analyses using transcriptome revealed that chemokine receptor-associated events are potently involved in metal-induced adverse actions. As an early sentinel of metal exposure, the precision-cut intestine slices (PCIS) induced the expression of chemokines including CXCLi1 or CXCLi2, which were potent gut-derived factors that activate chemokine receptors in reproductive organs after circulation.

Conclusion: EF-selective metals can be estimated to trigger the chemokine circuit in the gut-reproductive axis of chickens. This in vitro methodology using PCIS-EF culture could be used as a promising alternate platform for the reproductive immunotoxicological assessment.


Heavy metals; Embryonic fibroblasts; PCIS; Chemokine receptor
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