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Evaluation of the QuantaMatrix Multiplexed Assay Platform for Molecular Diagnosis of Multidrug- and Extensively Drug-Resistant Tuberculosis Using Clinical Strains Isolated in Myanmar

Annals of Laboratory Medicine 2020년 40권 2호 p.142 ~ 147
 ( Chang Yun-Hee ) - Yonsei University College of Health Science Department of Biomedical Laboratory Science

 ( Kim Seo-Yong ) - Yonsei University College of Health Science Department of Biomedical Laboratory Science
 ( Kim Yeun ) - Yonsei University College of Health Science Department of Biomedical Laboratory Science
 ( Ei Phyu Win ) - Yonsei University College of Health Science Department of Biomedical Laboratory Science
 ( Hwang Da-Som ) - Yonsei University College of Health Science Department of Biomedical Laboratory Science
 ( Lee Jong-Seok ) - International Tuberculosis Research Center
 ( Chang Chul-Hun L. ) - Pusan National University Yangsan Hospital Department of Laboratory Medicine
 ( Lee Hye-Young ) - Yonsei University College of Health Science Department of Biomedical Laboratory Science

Abstract


Background: Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. we developed QuantaMatrix Multiplexed Assay Platform (QMAP) MDR/XDR assay (QuantaMatrix Inc., Seoul, Korea) that can simultaneously detect mutations related to both first- and second-line drug resistance (rifampin, isoniazid, ethambutol, fluoroquinolones, second-line injectable drugs, and streptomycin).

Methods: We used 190 clinical Mycobacterium tuberculosis (MTB) strains isolated from Myanmar, compared QMAP and pDST results, and determined concordance rates. Additionally, we performed sequence analyses for discordant results.

Results: QMAP results were 87.9% (167/190) concordant with pDST results. In the 23 isolates with discordant results, the QMAP and DNA sequencing results completely matched.

Conclusions: The QMAP MDR/XDR assay can detect all known DNA mutations associated with drug resistance for both MDR- and XDR-MTB strains. It can be used for molecular diagnosis of MDR- and XDR-TB to rapidly initiate appropriate anti-TB drug therapy.

키워드

Mycobacterium tuberculosis; Multidrug-resistant tuberculosis; Extensively drug-resistant tuberculosis; QuantaMatrix Multiplexed Assay Platform
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