medric medric
[닫기]
잠시만 기다려 주세요. 로딩중입니다.

Upregulated Long Noncoding RNA LINC01234 Predicts Unfavorable Prognosis for Colorectal Cancer and Negatively Correlates With KLF6 Expression

Annals of Laboratory Medicine 2020년 40권 2호 p.155 ~ 163
 ( Jiang Xiao ) - Nanjing Medical University Wuxi People’s Hospital Department of Ultrasound

 ( Zhu Qiaoying ) - Nanjing Medical University Wuxi People’s Hospital Department of Ultrasound
 ( Wu Pengxi ) - Nanjing Medical University Wuxi People’s Hospital Department of Ultrasound
 ( Zhou Fengsheng ) - Nanjing Medical University Wuxi People’s Hospital Department of Ultrasound
 ( Chen Jun ) - Nanjing Medical University Wuxi People’s Hospital Department of Ultrasound

Abstract


Background: LINC01234, a long noncoding RNA (lncRNA), is overexpressed in several cancers, including colorectal cancer (CRC). We investigated the role of LINC01234 in CRC development and confirmed its correlation with Kruppel-like factor 6 (KLF6), a tumor suppressor gene that is dysregulated in CRC.

Methods: We tested mRNA levels using quantitative reverse transcription PCR (qRT-PCR). Tissue samples from patients with CRC, inflammatory bowel disease (IBD), hyperplastic polyp, and adenoma were included. Correlations between clinicopathological parameters, overall survival (OS) rate, and LINC01234 were analyzed using Kruskal-Wallis H test. Additionally, cell proliferation, apoptosis, and tumor formation in nude mice were tested to investigate the mechanism of LINC01234. Western blotting was used to determine protein levels.

Results: LINC01234 expression was significantly upregulated in CRC tissues and CRC cell lines than in non-tumor tissues and normal epithelial cells, respectively. LINC01234 was associated with high tumor stage, larger tumor size, and metastasis. Patients with higher LINC01234 expression showed reduced OS. Cell proliferation was inhibited by LINC01234 knockdown, whereas apoptosis was enhanced. Mice injected with SW480 cells with LINC01234 knockdown displayed decreased tumor volume, weight, and Ki-67 levels compared with those injected with control cells. KLF6 was negatively regulated by LINC01234. Overexpression of KLF6 showed effects similar to those observed following LINC01234 knockdown on cell proliferation and apoptosis.

Conclusions: LINC01234 could be a prognostic biomarker in CRC patients. Upregulation of LINC01234 in CRC promotes tumor development through negative regulation of KLF6.

키워드

Long noncoding RNA; LINC01234; Colorectal cancer; Kruppel-like factor 6; Prognosis
원문 및 링크아웃 정보
 
등재저널 정보