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모델 랫드에 간 허혈/재관류로 유발된 손상에 대한 항산화제의보호 효과에 관한 연구

A Study on the Protective Effect of Antioxidants on Damage Induced by Liver Ischemia/Repefusion in a Rat Model

대한임상검사과학회지 2019년 51권 3호 p.370 ~ 378
안용호 ( Ahn Yong-Ho ) - 동남보건대학교 임상병리과

석푸름 ( Seok Pu-Reum ) - 을지대학교 임상병리학과
오수진 ( Oh Su-Jin ) - 을지대학교 임상병리학과
최진우 ( Choi Jin-Woo ) - 을지대학교 임상병리학과
신재호 ( Shin Jae-Ho ) - 을지대학교 임상병리학과

Abstract


The hepatic ischemic model has recently been widely used for the epidemiological study of ischemic reperfusion injury. This study was carried out to investigate the protective effect of vanillin, which is known to have antioxidant and anti-inflammatory effects, against hepatic and renal injury using an ischemia-reperfusion rat model, and we also investigated the mechanism related to vanillins’ protective effect. The test material was administered at a concentration of 100 mg/kg for 3 days, followed by ligation of the liver for 60 minutes to induce ischemia reperfusion. As control groups, there was a negative control, sham control and ischemia-reperfusion-only ischemia reperfusion control, and the controls groups were compared with the drug administration group. In the vanillin group, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were significantly inhibited compared with the AST and ALT activities of the ischemia-reperfusion group, and histopathological examination showed significant reduction of both inflammation and necrosis. The malondialdehyde (MDA) and superoxide dismutase (SOD) levels were significantly different from the ischemia-reperfusion group. In conclusion, vanillin showed a hepatocyte protective action by alleviating the cellular inflammation and cell necrosis caused by hepatic ischemia-reperfusion, and vanillin mitigated inflammatory changes in the kidney glomeruli and distal tubules. The protective effect is considered to be caused by vanillin’s antioxidant function.
Further studies such as on cell death and possibly vanillin’s same effect on damaged tissue will be necessary for clinical applications such as organ transplantation.

키워드

Ischemia-reperfusion; Liver; Kidney; Rat; Vanillin
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