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Phage Promotro Base Paris Discriminatively Recognized by SP6 and T7 RNA Polymerases

Molecules and Cells 1992년 2권 1호 p.83 ~ 86
 ( Lee Sang-Soo ) - Korea Advanced Instituted of Science and Technology Department of Life Science

 ( Kang Changwon ) - Korea Advanced Instituted of Science and Technology Department of Life Science

Abstract


The phage SP6 and T7 RNA polymerases selectively recognize their own promoters, al-though both phage promoters share high-degree homology in base pair sequence. Recently we have demonstrated that changing the base pairs at -9 and -8 of the T7 consensus promoter sequence alone to SP6 promoter-specific base pairs switches the T7 promoter variant to an SP6 promoter [Lee, S. S., and Kang, C. (1992) Biochem. Intl 26, 1-51. For complementary study on SP6 promoter, the base pairs at -10, -9, -8 of the SP6 promo-ter consensus sequence were changed singly and multiply to the T7 promoter-specific base pairs and assayed for T7 and SP6 promoter activities. Changing only the -9 and -8 base pairs in the SP6 consensus promoter sequence to the T7 promoter-specific base pairs enables transcription by T7 RNA polymerase, but reducing the SP6 promoter activity substantially. Thus, these results confirm and support our previous finding that the -9 and -8 base pairs in both SP6 and T7 promoters are responsible for the distinction between SP6 and T7 promoters.

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