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대장암 세포주에서 Deoxycholate노출후 세포내 Glutathione S-Transferase의 변화

Cytosolic Glutathione S-Transferase Change after Deoxycholate Exposure in Colon Cancer Cell Lines

대한대장항문학회지 1998년 14권 4호 p.701 ~ 707
박동국 ( Park Dong-Guk ) - 단국대학교 의과대학 외과학교실

신지현 ( Shin Ji-Hyun ) - 단국대학교 의과대학 소화기내과학교실
박석건 ( Park Seok-Gun ) - 단국대학교 의과대학 핵의학교실
정선용 ( Jung Sun-Young ) - 단국대학교 의과대학 핵의학교실

Abstract


Purpose: Bile acids (especially deoxycholate) was known to be toxic and mutagenic on colon epithelium. They proposed at least four mechanisms for the bile acid toxicity. It is the one of these mechanisms that bile acid inhibits the xenobiotic metabolizing enzyme activity (esp glutathione S-transferase, GST). So we measured the cytosolic GST level of colon carcinoma cell lines after deoxycholate exposure whether or not the deoxycholate lowered the cytosolic GST activity.

Methods: Three colon cancer cell lines (LoVo, SW480, HT29) were used for this study. We calculated the cellular toxicity by MTS method. And cytosolic GST activity was measured according to the method as Habig described. For total GST activity, 2.5 mM 1-chloro-2,4-dinitrobenzene was used for substrate, and measured as absorbance in 340 nm.

Results: Basal cytosolic GST level for LoVo, SW480, HT29 cell line was 514.59± 27.01, 291.63±38.44 and 344.58±47.92 nmol/min/mg cytosol protein. GST level did not changed significantly after 5 days culture without DCA. But GST level was decreased significantly to 128.63±21.35, 134.33±41.76 and 163.10±22.73 nmol/min/mg cytosol protein each cell line after 5 days deoxycholate exposure (p<0.005).

Conclusion: Cytosolic GST level was lowered significantly after deoxycholate exposure for 5 days. One of the mechanisms of bile acid toxicity for colon cancer cell is proposed to inhibit cytosolic GST activity.

키워드

Glutathione S Transferase;Deoxycholate;Colon cancers
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